Addgene

We narrowed to 11,654 results for: cel.2

Showing: 3781 - 3810 of 11654 results

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D15_Ld_061240

Plasmid

#135831

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

thioredoxin, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D13_Ld_050900

Plasmid

#135829

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

surface antigen- like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D12_Ld_040370

Plasmid

#135828

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D11_Ld_211610

Plasmid

#135827

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D6_Ld_100150

Plasmid

#135822

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

nucleoside phosphatase, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D65_Ld_051210

Plasmid

#135878

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

surface antigen-like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D3_Ld_030930

Plasmid

#135819

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

multicopper oxidase, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D1_Ld_290860

Plasmid

#135817

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

cysteine peptidase C (CPC)

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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D10_Ld_292250

Plasmid

#135826

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

C-8 sterol isomerase-like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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D17_Ld_090870

Plasmid

#135833

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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D8_Ld_340400

Plasmid

#135824

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, unknown function

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR-ADAM29i2

Plasmid

#59607

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_WW34

Plasmid

#187718

Purpose

Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-581 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

only residues 492-581

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_HECT

Plasmid

#187719

Purpose

Express GST-tagged NEDD4L HECT domain in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 594-975 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-593

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2-RNA-MUT

Plasmid

#238978

Purpose

Expresses the RNA-binding-deficient mutant of YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-RNA-MUT (YTHDF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

RNA binding mutation (K416A+R527A)

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2-D-MUT

Plasmid

#238977

Purpose

Expresses the domain-mutant YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-D-MUT (YTHDF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

5 site mutation (W432A+W486A+Y418A+D422A+W491A)

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)

Plasmid

#232945

Purpose

Expresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdown

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2 (YTHDF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

synonymous mutation

Promoter

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.0

Plasmid

#208301

Purpose

Expresses ABE-RA3.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)

Plasmid

#190746

Purpose

Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pETduet+p300HAT

Plasmid

#157793

Purpose

Expression of p300 HAT domain

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

p300HAT (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSR646

Plasmid

#65215

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-6 cap

Use

AAV

Tags

Expression

Insect

Mutation

Promoter

P10 and polyhedrin

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 WT

Plasmid

#123103

Purpose

Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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hKDR

Plasmid

#83436

Purpose

human VEGFR2 with V5 and tGFP tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

Insert

human VEGFR2 (KDR Human)

Use

Tags

V5 and turboGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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