We narrowed to 6,450 results for: siae
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Plasmid#87292PurposeEncodes Y2H Gal4 activation domain for Hsh155 R294CDepositorInsertHSH155 (HSH155 Budding Yeast)
TagsGAL4 AD and HAExpressionBacterial and YeastMutationR294CAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 E291D
Plasmid#87290PurposeEncodes Y2H Gal4 activation domain for Hsh155 E291DDepositorInsertHSH155 (HSH155 Budding Yeast)
TagsGAL4 AD and HAExpressionBacterial and YeastMutationE291DAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/K335E
Plasmid#87286PurposeEncodes Hsh155 N295D K335E for expression in yeastDepositorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/D450G
Plasmid#87284PurposeEncodes Hsh155 R294L D450G for expression in yeastDepositorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/K335E
Plasmid#87283PurposeEncodes Hsh155 R294L K335E for expression in yeastDepositorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
ExpressionBacterial and YeastPromoterpGPD1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
TagsVenusExpressionBacterial and YeastPromoterpSTL1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS425-M25-Afg1
Plasmid#61720Purposeyeast Afg1 AAA protein in yeast expression vectorDepositorInsertAfg1 (AFG1 Budding Yeast)
ExpressionYeastAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS13-Pleum
Plasmid#60332PurposeContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceMay 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
UASGAL-CU1-Pleum
Plasmid#60331PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)
Plasmid#52896PurposeSkn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSkn7t-L-IFP-F[1]
TagsL-IFP-F[1]ExpressionYeastPromoterGAL1Available SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA5
Plasmid#46791PurposeTo express a version of Fun30 without CUE domain in budding yeastDepositorAvailable SinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA12
Plasmid#46792PurposeTo express a version of Fun30 with a mutated CUE domain in budding yeastDepositorAvailable SinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH738-CENBEVYslow
Plasmid#38220DepositorInsertGCN4-based single uORF-containing 5'-UTR
ExpressionYeastMutationThe start codons of uORFs2, 3 and4 of the yeast G…PromoterTDH3 (=GPD)Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLG-Nde-Acc
Plasmid#33150DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
TagsLacZExpressionYeastMutationAccI site in intron was cut/filled/ligated to cha…Available SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pRS416 Gal-RNQ1 L94A-YFP
Plasmid#18692DepositorInsertRnq1 L94A (RNQ1 Budding Yeast)
TagsYFPExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)Available SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys
Plasmid#15602DepositorInsertSup35 NM (SUP35 Candida albicans, Budding Yeast)
Tags7xHis and CysteineExpressionBacterialMutation4 G/A. Four glycines mutated to alanines. The pos…Available SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pG1-NMGR526(d22-69)
Plasmid#1125DepositorTagsGR526ExpressionYeastMutationnucleotides encoding NM domain amino acids 22-69 …Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35N-KDG6-C
Plasmid#1238DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidMutationSUP35 middle region (aa124-253) was replaced with…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…Available SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-NatMX6
Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYSD099
Plasmid#212923PurposeA MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.DepositorInsertGFP
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsExpressionBacterialPromoterPgyrAAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK419 (GAL-OsTIR1(F74G))
Plasmid#140656PurposeGAL-OsTIR1(F74G)DepositorInsertGAL-OsTIR1(F74G)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only