We narrowed to 5,312 results for: cycs

1. pcDNA5-FRT/T0-Flag-53BP1

   Plasmid

   #52507

   Purpose

   mammalian expression vector of 53BP1

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V128A and siRNA resistant sequence: ACGCGCTGACGAC…

   Promoter

   CMV/TO

   Available since

   March 26, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. EXO1B-AcGFP

   Plasmid

   #215125

   Purpose

   Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   EXO1B (EXO1 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-BLAST flag-CAD S1859A

   Plasmid

   #169821

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859A; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1 KBTBD4 WT

   Plasmid

   #184624

   Purpose

   Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

   Plasmid

   #113694

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-NR2F1

   Plasmid

   #170698

   Purpose

   doxycycline-inducible overexpression of NR2F1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   deletion of 36G- please see depositor comments

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-3xFLAG-RSL1D1(WT)

   Plasmid

   #122301

   Purpose

   expresses RSL1D1 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459-AMBRA1 #3

   Plasmid

   #172606

   Purpose

   Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p(HA)HIF1alpha(401delta603)R27G

    Plasmid

    #52215

    Purpose

    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

    Depositor

    Eric Huang

    Article

    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

    Insert

    HIF1alpha (401delta603) R27G (HIF1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 401-603 deleted, removes the oxygen-dependent …

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid

    #187276

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaActin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSICO-CPSF6-4Glu-iRFP670

    Plasmid

    #167588

    Purpose

    Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-4Glu-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    K547E, R549E, R559E, R561E

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid

    #187275

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaMyosin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mKO2-CDK4

    Plasmid

    #215065

    Purpose

    Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-FH-AGO2-5xA

    Plasmid

    #91979

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-Puro-FH-AGO2-5xE

    Plasmid

    #91993

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113687

    Purpose

    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1-FH-AGO2-5xE

    Plasmid

    #91981

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113688

    Purpose

    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-Puro-FH-AGO2-5xA

    Plasmid

    #91992

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge