We narrowed to 3,452 results for: ttl

1. pYPQ265O1

   Plasmid

   #173999

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   D10A

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox

   Plasmid

   #182360

   Purpose

   Shuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2

   Depositor

   Mario Capecchi

   Insert

   Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Adeno-miR-23b

   Plasmid

   #112538

   Purpose

   Adenovirus overexpression

   Depositor

   Shusheng Wang

   Article

   Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

   Insert

   miR-23b (MIR23B Human)
   

   Use

   Adenoviral

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6DUB (LOC149281)

   Plasmid

   #119967

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   LOC149281 (METTL11B Human)
   

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 58-278

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOEM1_pCMV:axFgf8-pPH:vsvged

   Plasmid

   #111159

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Nacu et al Nature. 2016 May 19;533(7603):407-10. do

   Insert

   axFgf8
   

   Use

   Baculoviral rescue shuttle vector

   Expression

   Insect and Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS2-aurZ

   Plasmid

   #70151

   Purpose

   Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   aurZ
   

   Expression

   Yeast

   Promoter

   Tef

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS3-aurJ

   Plasmid

   #70152

   Purpose

   Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   aurJ
   

   Expression

   Yeast

   Promoter

   GPD1

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Promoter

   tac and unknown

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJUMP24_T24_pRham-ilvA-relE

   Plasmid

   #201531

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE
   

   Use

   Synthetic Biology

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEC2861 (pSpy1C-PgyrA_ffluc)

    Plasmid

    #218511

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PgyrA

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK02

    Plasmid

    #105133

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX-TRV2

    Plasmid

    #180516

    Purpose

    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker

    Depositor

    Jose-Antonio Daros

    Article

    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

    Insert

    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    

    Expression

    Plant

    Promoter

    35S CaMV

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFW1000

    Plasmid

    #224210

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p201H Cas9

    Plasmid

    #59176

    Purpose

    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    hph
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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