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PB-SRT-Puro_BC2

Plasmid

#193144

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Expression

Mammalian

Mutation

Barcode: CTGA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro_BC1

Plasmid

#193143

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Expression

Mammalian

Mutation

Barcode: CACA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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XLone-BSD NFIL3

Plasmid

#179512

Purpose

Tunable and temporal expression control of NFIL3

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

NFIL3 (NFIL3 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS Promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Plasmid

#174641

Purpose

Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-u(XXL)Cas9

Plasmid

#127387

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XXL)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc C1A4E

Plasmid

#141088

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2

Plasmid

#124063

Purpose

Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXIATL2

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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zebrafish SLC2A2 (zfSLC2A2)

Plasmid

#65103

Purpose

Full length SLC2A2 cDNA (Danio rerio)

Depositor

Josep Planas

Article

Castillo et al Am J Physiol Regul Integr Comp Physiol.

Insert

SLC2A2 (slc2a2 Zebrafish)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

Plasmid

#112803

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 554_557 deleted/PDZ

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22)

Plasmid

#112800

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 deleted

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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p1G108CFa

Plasmid

#85089

Purpose

Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to an FAD domain of P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1, A74G/C773S/C810S in FAD domain of…

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1241

Plasmid

#29123

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple12

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-eGFP

Plasmid

#182294

Purpose

Fluorescent reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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