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pDONR223-PRKAG3

Plasmid

#23549

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG3 (PRKAG3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSUPER.MRE11i

Plasmid

#15566

Depositor

Didier Trono

Publication

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

RNAi

Expression

Mammalian

Available since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
piGECI-N1

Plasmid

#160421

Purpose

Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Publication

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Publication

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Expression

Bacterial

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPBbsr-JNK KTR-mCherry

Plasmid

#115493

Purpose

Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

JNK KTR-mCherry

Tags

mCherry

Expression

Mammalian

Mutation

mCherry G229L, mCherry Y298 deleted

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p3xFlag-CMV7-Erk2_KR

Plasmid

#39224

Depositor

Melanie Cobb

Publication

Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

Insert

Erk2(K52R) (Mapk1 Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

K52R

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p231 pCMVe-betaAc-STOP-luc

Plasmid

#12463

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc-STOP

Use

Luciferase

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pUC57-Curlcake3

Plasmid

#139342

Purpose

Expresses Curlcake3 (CC3) in E.coli

Depositor

Eva Maria Novoa

Publication

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake3 with T7 promoter

Expression

Bacterial

Promoter

T7

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR - CTRL sgRNA

Plasmid

#70662

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Publication

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDC-UAS-nBitbow1.0(miniW)

Plasmid

#138329

Purpose

Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

Depositor

Dawen Cai

Publication

Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

Inserts

mAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2

Tags

human H2B

Expression

Insect

Promoter

10xUAS

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Publication

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mouse-DJ1-HA

Plasmid

#29396

Depositor

Mark Cookson

Publication

Miller et al Neurosci Lett. 2005 Feb 21. 374(3):203-6

Insert

DJ1 (Park7 Mouse)

Tags

HA

Expression

Mammalian

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pAM5411

Plasmid

#132663

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Publication

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAM5409

Plasmid

#132662

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Publication

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAVS1-Puro cTnT FUCCI

Plasmid

#136935

Purpose

donor plasmid for cardiac-specific FUCCI expression in human cells

Depositor

Xiaoping Bao

Publication

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Troponin T promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

cTnT

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLgw-MBD3-V5-Dam

Plasmid

#55605

Purpose

Expression of Dam fused MBD3 in mammalian cells

Depositor

Paul Wade

Publication

Shimbo et al PLoS Genet. 2013 Dec;9(12):e1004028. doi

Insert

MBD3 (MBD3 Human)

Use

Lentiviral

Tags

DamID (DNA adenine methyltransferase identificati…

Expression

Mammalian

Promoter

HSP

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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