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Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpresses tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVExpressionMammalianPromoterCMVAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-CEN_ARS_HIS
Plasmid#202129PurposeLevel 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.DepositorInsertCEN_ARS_HIS
UseSynthetic BiologyMutationARS sequence mutated to remove sapI site for dome…Available SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
piGECI-NES-N1
Plasmid#160422PurposeExpression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequenceDepositorInsertiGECI-NES
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2
Plasmid#48742PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +386)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-JEDI-1P-WPRE
Plasmid#202616PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAPDepositorInsertJEDI-1P
UseAAVExpressionMammalianPromoterGFAPAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCV-Rep-
Plasmid#32058DepositorInsertMCV-Rep- genome
MutationSingle cytosine to adenine (C/A) mutation at 2,07…Promoternone from vector. Insert contains native promoter…Available SinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-UAG site)
Plasmid#170138PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(UAG)-MS2
UseAAVExpressionMammalianPromoterHuman U6Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVExpressionMammalianPromoterHuman U6Available SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA1
Plasmid#172709PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA2
Plasmid#172710PurposeCas9 with 3' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AQTFAATHNPWASQA_epitope
Plasmid#165019PurposeExpression of CMV UL86 (1222-1236) fused to CD74DepositorInsertCMV UL86 (1222-1236)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianPromoterCMVAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)
Plasmid#87735PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GSX2-tTA-BleoR
Plasmid#161748PurposeTet-ON tTA plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertAdvanced tTA
UseCRISPRExpressionMammalianPromoterendogenous GSX2 promoterAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330-UFSP2 sgRNA2
Plasmid#134637Purposecontains sgRNA targeting human UFSP2 for gene knockoutDepositorInsertUFSP2 sgRNA2 (UFSP2 Human)
ExpressionMammalianAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-3
Plasmid#193696PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL1
Plasmid#48741PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to -141)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
hGR-Dx
Plasmid#133408Purposeoverexpression of a mutant human glucocorticoid receptor in mammalian cellsDepositorAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Lyn-Y-GECO1
Plasmid#58200PurposeExpress the membrane targeted Y-GECO1 in neuronsDepositorInsertY-GECO1.0
UseAAVPromoterhSynAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only