We narrowed to 12,966 results for: lic

1. pGEMHE:mTREK-1(G137I)

   Plasmid

   #133272

   Purpose

   X. laevis expression vector. It will generate the mouse TREK-1 channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
   

   Use

   Oocyte expression

   Mutation

   G137I

   Promoter

   T7

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Amber-free HIV-1 deltaRT

   Plasmid

   #213007

   Purpose

   An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

   Depositor

   Maolin Lu

   Article

   Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

   Inserts

   HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
   Env BG505
   

   Use

   Unspecified

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMK ENTR 2 hU6 ACTB sgRNA

   Plasmid

   #206270

   Purpose

   ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   ACTB sgRNA
   

   Use

   CRISPR; Multimate/gateway entr 2

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMK ENTR 2 hU6 HEK3 PegRNA

   Plasmid

   #206277

   Purpose

   ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HEK3 PegRNA
   

   Use

   CRISPR; Multimate/gateway entr 2

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMK ENTR 3 polH aeBlue polH VSV-G

   Plasmid

   #206278

   Purpose

   ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   aeBlue, VSV-G
   

   Use

   Multimate/gateway entr 3

   Expression

   Bacterial and Insect

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.0-TERT_-100_-79

   Plasmid

   #193785

   Purpose

   luciferase reporter for duplicated TERT promoter

   Depositor

   Joseph Costello

   Article

   Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   TERT

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDA2ScIfm

   Plasmid

   #193786

   Purpose

   Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTATAA synthetic promoter carrying binding sites…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLA2ScIfmTcIfm

    Plasmid

    #193792

    Purpose

    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLLacO-1 and PLTetO-1

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. deltaVP1d63C + VP2C-GFPdeg

    Plasmid

    #182481

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFPdeg
    deltaVP1d63C
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Linked VLP NES (M)

    Plasmid

    #182434

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Linked VLP NES (G)

    Plasmid

    #182476

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

    Plasmid

    #182918

    Purpose

    Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    pH2B (H2BC16P Human)
    

    Tags

    mNeonGreen-FRB(DmrC)

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-D-kcnj1b

    Plasmid

    #173126

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-kcnj8

    Plasmid

    #173134

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIG1_UL136_26kDa_Myc

    Plasmid

    #74955

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

    Expression

    Mammalian

    Mutation

    delta (1-62) M78A M80A M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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