We narrowed to 7,798 results for: ttl

Showing: 2541 - 2560 of 7798 results

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AD-003

Plasmid

#25502

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

AD-003 (NTMT1 Human)

Tags

6x His and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pJYDN3

Plasmid

#162456

Purpose

Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

Depositor

Gideon Schreiber

Publication

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGTag-P2A-QFGal4-SV40pA

Plasmid

#155127

Purpose

Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P2A-QFGal4

Use

CRISPR and Synthetic Biology

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

Plasmid

#136382

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-VPR

Use

CRISPR

Expression

Plant

Mutation

D570A

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p3E_BAG3P209L-eGFP

Plasmid

#98181

Purpose

Codes for full length, GFP tagged mutant Bag3

Depositor

Robert Bryson-Richardson

Publication

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BAG3P209L-eGFP (BAG3 Human)

Use

Gateway vector

Tags

GFP

Mutation

P209L

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-3x125bp-mnx1

Plasmid

#169043

Purpose

5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

Depositor

Paola Arlotta

Publication

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

Mnx1 enhancer (3x) (mnx1 Zebrafish)

Use

Gateway entry cloning vector

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-dzCas9-NG-Act3.0

Plasmid

#158415

Purpose

CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP-NIK

Plasmid

#111208

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Publication

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIK (MAP3K14 Human)

Tags

EYFP

Expression

Mammalian

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX305-N-dTAG-CARD8

Plasmid

#169989

Purpose

Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

dTAG

Expression

Mammalian

Promoter

hPGK

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pIB164

Plasmid

#90187

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

Depositor

Indranil Biswas

Publication

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCM1.120

Plasmid

#19600

Depositor

Geraldine Seydoux

Insert

spn-4 prom in PDONRP4P1R

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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791 p3E-2A-pCS2MCSpA v2

Plasmid

#195973

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-multiple cloning site

Use

Other

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEN41A-T38S38

Plasmid

#49524

Purpose

Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

Depositor

Dirk Schnappinger

Publication

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Promoter

Ptb38

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRG215

Plasmid

#64525

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCM1.108

Plasmid

#21382

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

him-3 prom in PDONRP4P1R

Use

Gateway Cloning

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pCM5.44

Plasmid

#19389

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

cep-1 3'utr in pDONRP2RP3

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway Cloning

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCM1.157

Plasmid

#21388

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

gld-1/cye-1 operon in pDONRP2RP3

Use

Gateway Cloning

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pIB169

Plasmid

#90192

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Publication

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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