We narrowed to 6,038 results for: WUS;

1. AAV_Actb HR donor

   Plasmid

   #97317

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-haA3A-CBE-VA

   Plasmid

   #220893

   Purpose

   Express haA3A-CBE-VA (with SpCas9) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-VA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-haA3A-CBE-VA-NG

   Plasmid

   #220896

   Purpose

   Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-VA-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgACSL4

   Plasmid

   #186024

   Purpose

   knock out ACSL4 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hAID-BE3

   Plasmid

   #113417

   Purpose

   Expresses hAID-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hAID-BE3 (AICDA S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-NES-NIR-GECO1

   Plasmid

   #113683

   Purpose

   Express NIR-GECO1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NES-NIR-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CMV-mouse Gfi1-GFP

   Plasmid

   #91892

   Purpose

   3rd generation lentiviral expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Unknown

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Tox2-IRES-eGFP

   Plasmid

   #165428

   Purpose

   Expresses TOX2 in mammalian cells

   Depositor

   Patrick Hogan, Anjana Rao

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX2 (Tox2 Mouse)
   

   Use

   Tags

   3xflag 5' of Tox2

   Expression

   Mammalian

   Mutation

   Promoter

   5' LTR

   Available since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T1_GST-PLproCS-MBP

   Plasmid

   #169195

   Purpose

   To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   GST-PLproCS-MBP
   

   Use

   Tags

   GST-MBP fusion (PLpro-cleavable) and His6 (intern…

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-Spy-CD86

    Plasmid

    #223598

    Purpose

    Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. JWW-2 human chimeric monoclonal antibody

    Plasmid

    #66749

    Purpose

    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-2 Heavy Chain
    JWW-2 Light Chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 and rEF1

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p23-NES-AdmCas13d-msfGFP-NES-Flag

    Plasmid

    #165075

    Purpose

    overexpression of AdmCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    AdmCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Puro-mGSDME F2A

    Plasmid

    #154878

    Purpose

    Expresses mGSDME F2A in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changes Phenylalanine 2 to Alanine

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.15_pSOX17

    Plasmid

    #195496

    Purpose

    Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Promoter region of SOX17 (SOX17 Human)
    

    Use

    Tags

    luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-TetO-Tead4

    Plasmid

    #128832

    Purpose

    dox-dependent expression of mouse Tead4

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Tead4 (Tead4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP

    Plasmid

    #180792

    Purpose

    Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLA (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Y226F, Y243F, Y257F, Y282F

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-BEACON2

    Plasmid

    #171698

    Purpose

    Expresses BEACON2 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W98Y/W104A/Y130F

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P2-MDMX (23-111)

    Plasmid

    #62064

    Purpose

    Expression of human MDM4 (residues 23-111) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 23-111

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

    Plasmid

    #170226

    Purpose

    Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    IKBKB (IKBKB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S177E, S181E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

    Plasmid

    #78606

    Purpose

    pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    173CMV, U6

    Available since

    Dec. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

22. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-HAT1_AG

    Plasmid

    #217113

    Purpose

    Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HAT1_AG transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiCRISPR v2 puro hGSDME gRNA2

    Plasmid

    #223520

    Purpose

    Knocking out hGSDME in human cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR1A-TAZ S89A

    Plasmid

    #179911

    Purpose

    Entry vector of TAZ S89A for gateway coloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Entry vector

    Tags

    3X Flag-tag

    Expression

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLVX-SV40-Galpha-s ONE-GO

    Plasmid

    #204327

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV_Actb NHEJ donor

    Plasmid

    #97316

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRetroX-mCRT-GPI-IRES-DsRed

    Plasmid

    #223711

    Purpose

    Mammalian expression of mouse CRT-GPI

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin-GPI (Calr Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

    Plasmid

    #187578

    Purpose

    Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    WRAP53 (WRAP53 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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