We narrowed to 10,761 results for: T7
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pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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NmMetQ WT
Plasmid#129647PurposeExpresses the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
Tags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialPromoterT7 PromoterAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-FAK
Plasmid#74478PurposeThis construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.DepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pZS*11-yfp0
Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
ExpressionBacterialPromoterPLtetO1Available SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-trAtCCD8(pYL736)
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hXRCC1
Plasmid#52283PurposeBacterial expression of human XRCC1 C-terminally fused to GyrA intein and GSTDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK220
Plasmid#71123PurposeExpression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coliDepositorInsertpantothenate kinase
TagsH6ExpressionBacterialMutationArg175 is mutated to a HisPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC05
Plasmid#114684PurposeFKBP-TEV-CDepositorInsertFKBP-TEV-C
TagsGSTExpressionBacterialPromoterT7 PromoterAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperPromoterT7Available SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-C. thermophilum LIC
Plasmid#74780Purposeencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilumDepositorInsertdynein light intermediate chain (Chaetomium thermophilum)
TagsGSTExpressionBacterialPromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SAR1
Plasmid#67388PurposeExpression of native hSar1 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-clbB
Plasmid#114155PurposeExpresses full-length ClbBDepositorInsertclbB
Tags6xHis-thrombin-T7 tagExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisMutationE275G, I287V and R294GPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pETx-SR6v15.d
Plasmid#176213PurposeBacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15.d
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GST-05230
Plasmid#67874PurposeE.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.DepositorInsertshy1
TagsGSTExpressionBacterialPromoterT7Available SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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knob-s48i48
Plasmid#68391PurposeKnob protein fused to elastin-like polypeptide (S48I48)DepositorInsertKnob-S48I48
TagsKnobExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28b-pchA WT
Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15.t
Plasmid#176214PurposeBacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15.t
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hTDG-K330A
Plasmid#52282PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GSTDepositorInserthTDG (TDG Human)
TagsGyrA-intein-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330)
Plasmid#87738PurposeBacterial expression of the VSL domain of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-279PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_H303A
Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDiySpike2
Plasmid#136949PurposePlasmid for the In Vitro transcription of DiySpike2DepositorInsertDiySpike2
UseSynthetic BiologyPromoterT7Available SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only
