We narrowed to 42,583 results for: cha;

1. pCAGGS-FLAG-BMAL1-S519A

   Plasmid

   #186834

   Purpose

   Expresses FLAG-BMAL1-S519A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 519 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

   Plasmid

   #112496

   Purpose

   Expresses channel rhodopsin in astrocytes

   Depositor

   Baljit Khakh

   Article

   Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

   Insert

   ChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   H134R

   Promoter

   GfaABC1D

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKS913.CB18.31

   Plasmid

   #69569

   Purpose

   TCR beta chain genomic transgenic construct for creating OT-1 mice

   Depositor

   Francis R. Carbone

   Article

   Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

   Insert

   TCR beta (Tcrb Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDN-D2irTN2AG5kwh

   Plasmid

   #128253

   Purpose

   Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   Beta Globin Intron::hTetR::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV D2ir promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEos2-Alpha-Actinin-19

   Plasmid

   #57346

   Purpose

   Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Alpha-Actinin (ACTN1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. BAS292 C terminal SF-GFP kanR

   Plasmid

   #74087

   Purpose

   S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   SF-GFP recoded for S.pombe
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pRF+275Dux4

   Plasmid

   #21293

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Apr 9. ():.

   Insert

   DUX 4 (DUX4 Human, DUX 4)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

8. PSUC2-yEGFPCLN2PEST-pRS406

   Plasmid

   #64405

   Purpose

   SUC2 promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   SUC2 promoter (SUC2 Budding Yeast)
   

   Use

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Mutation

   Promoter

   SUC2

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BAS287 C terminal E2C hygBR

   Plasmid

   #74082

   Purpose

   S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   E2Crimson
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pMXs-Trib3

    Plasmid

    #131606

    Purpose

    expression of mouse Trib3 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Trib3 (Trib3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PH-miRFP-FRB

    Plasmid

    #193568

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Use

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs_FLAG-ODC1

    Plasmid

    #133256

    Purpose

    Express FLAG-tagged ODC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC1 (ODC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBL_DK193

    Plasmid

    #156424

    Purpose

    Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (Q57H)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Q57H

    Promoter

    Polyhedrin

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB3020(gRNA X-2)

    Plasmid

    #73282

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCfB3042(gRNA X-4)

    Plasmid

    #73284

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFS485

    Plasmid

    #89348

    Purpose

    Leu1 integrating plasmid with cdc25 genomic fragment

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    cdc25 (cdc25 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-R2ACR_142-EYFP

    Plasmid

    #103964

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R2ACR_142
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ZJOM108

    Plasmid

    #133665

    Purpose

    Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA6 MCV LTco S147A

    Plasmid

    #112192

    Purpose

    Expression of MCPyV LT mutant (Ser 147 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs-Bex2

    Plasmid

    #131607

    Purpose

    expression of mouse Bex2 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Bex2 (Bex2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSK226

    Plasmid

    #129268

    Purpose

    dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Rnq1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCfB3047(gRNA XII-1)

    Plasmid

    #73289

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-D2ir promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRISPR01-FnCpf1

    Plasmid

    #176529

    Purpose

    Nuclease Cpf1 adapted for Aspergillus species

    Depositor

    Kusol Pootanakit

    Article

    Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

    Insert

    FnCpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. UASGAL-CU2-Pleum

    Plasmid

    #60328

    Purpose

    Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. M-ST1-VPR

    Plasmid

    #68498

    Purpose

    nuclease competent ST1-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    ST-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Promoter

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. UASGAL-PCYC

    Plasmid

    #60335

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pCfB3046(gRNA XI-5)

    Plasmid

    #73288

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfB3044(gRNA XI-2)

    Plasmid

    #73286

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfB3048(gRNA XII-2)

    Plasmid

    #73290

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mEos2-Alpha-Actinin-C-14

    Plasmid

    #57347

    Purpose

    Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pMS1088

    Plasmid

    #80159

    Purpose

    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

    Depositor

    Marcelo Sousa

    Article

    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

    Insert

    HisSUMO-Cys-4xNuG2-Ald
    

    Use

    Tags

    HisSUMO

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMM0647

    Plasmid

    #129008

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge