We narrowed to 21,628 results for: ESC

1. pAAV-CAG-eLACCO1.1

   Plasmid

   #168789

   Purpose

   Fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDUAL_2_araBAD

   Plasmid

   #155176

   Purpose

   see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKO2-SLBP(18-126)

   Plasmid

   #83914

   Purpose

   Fluorescent probe for S/G2 cell cycle transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mKO2-SLBP(18-126) (SLBP Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046828

   Plasmid

   #145742

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HiFi dCas9 VPR

   Plasmid

   #188510

   Purpose

   Expresses FLAG tagged HiFi dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMRE161

   Plasmid

   #118509

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-CAG_3C-Twin-Strep

   Plasmid

   #113901

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LbdCas12a VPR

   Plasmid

   #188513

   Purpose

   Expresses FLAG tagged LbdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRE164

    Plasmid

    #118512

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pSCMV-H2B-FT-Slow

    Plasmid

    #157671

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Slow-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCMV-H2B-FT-Fast

    Plasmid

    #157669

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Fast-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Perox-B

    Plasmid

    #209867

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHLuc Control

    Plasmid

    #141091

    Purpose

    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKRaION1-N1

    Plasmid

    #177801

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-iBB-GECO1

    Plasmid

    #194215

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

    Depositor

    Jin Zhang

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-eLACCO1.1

    Plasmid

    #168788

    Purpose

    Neuronal expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge