We narrowed to 3,170 results for: GRI;

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PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229L-pET151

Plasmid

#177879

Purpose

Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2-P229S-pET151

Plasmid

#177871

Purpose

Expresses mouse Talin 1 F2-P229S domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 P229S mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2-P229L-pET151

Plasmid

#177876

Purpose

Expresses mouse Talin 1 F2-P229L domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 P229L mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229S-pET151

Plasmid

#177878

Purpose

Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1

Plasmid

#187010

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

Plasmid

#174646

Purpose

Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

CMV (with TetOn)

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-mVenus-SSPB(micro)

Plasmid

#174635

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(micro)

Plasmid

#174627

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R

Plasmid

#166810

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL1193EE, P1214R

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

Plasmid

#166805

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL193EE

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

Plasmid

#166808

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K1277E

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

Plasmid

#166807

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H1276G

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE

Plasmid

#166797

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

RK96EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE

Plasmid

#166799

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL102EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N

Plasmid

#166802

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

D808N

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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