We narrowed to 10,458 results for: T7

1. pD(P+T)_T7

   Plasmid

   #223854

   Purpose

   4G-cloning donor for bacterial promoter and terminator, no tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PE-SUMO CRYGA

   Plasmid

   #217672

   Purpose

   For expression of SUMO tagged human gamma A crystallin

   Depositor

   Larry David

   Insert

   CRYGA (CRYGA Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8GCY

   Plasmid

   #214440

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBLB:PBC020-G02:C248501 (CBLB Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1_EVD68_3ABC_C147R

   Plasmid

   #212978

   Purpose

   Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Cysteine 147 in 3C to Arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7CFE1_EVD68_3ABCD_C147R

   Plasmid

   #212980

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 147 from 3C to arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7CFE1_EVD68_VP12A_N84T

   Plasmid

   #212983

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A N84T
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Asparagine 84 from 2A to Threonine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-Flag-RBM24-1~93

   Plasmid

   #208633

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 1~93, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-Flag-RBM24-95~236

   Plasmid

   #208634

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 95~236, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET24a_thyA_R166Q_untagged

    Plasmid

    #191307

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R166Q TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET24a_thyA_Q33S_untagged

    Plasmid

    #191308

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q33S TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET24a_thyA_R127A_untagged

    Plasmid

    #191309

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R127A TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a_thyA_WT_untagged

    Plasmid

    #191306

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. human KANK2 Ankyrin Repeats pET151

    Plasmid

    #164054

    Purpose

    Expresses human KANK2 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJC20_cam1

    Plasmid

    #182426

    Purpose

    Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    cam1 (cam1 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid

    #169403

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid

    #169404

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 13 pCOLI_A_CBP

    Plasmid

    #160155

    Purpose

    N-term CBP 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    CBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 29 pCOLI_S_C_STREP

    Plasmid

    #160171

    Purpose

    C-term 2xStrepII uncleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 27 pCOLI_S_C_HIS

    Plasmid

    #160169

    Purpose

    C-term 8xHis uncleavable Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge