We narrowed to 5,474 results for: chia

1. pET21a - Ec-ClpX

   Plasmid

   #111516

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB533-44B12m23-sfGFP

   Plasmid

   #186778

   Purpose

   RNAP2 Ser5ph-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   44B12m23-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCB306-GFP-Rab12

   Plasmid

   #198471

   Purpose

   lentiviral expression of GFP-Rab12

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   GFP-Rab12 (RAB12 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Plasmid EUPp_09

   Plasmid

   #157745

   Purpose

   PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

   Depositor

   Kang Zhou

   Article

   Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

   Inserts

   acetaldehyde dehydrogenase
   alcohol dehydrogenase (ADH2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PgyrA

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDTAI

   Plasmid

   #51627

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDR111_Bgal no NLS

   Plasmid

   #188397

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Phyper spank

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

    Plasmid

    #110559

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTA-Mob 2.0

    Plasmid

    #149662

    Purpose

    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

    Depositor

    Bogumil Karas

    Article

    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC035 - dLwCas13a-msfGFP

    Plasmid

    #91925

    Purpose

    Expresses catalytically inactive LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS-3xHA and NLS-msfGFP

    Expression

    Mammalian

    Mutation

    R488A and R1060A

    Promoter

    EF-1a

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNH-TrxT-G-L11

    Plasmid

    #127831

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

    Plasmid

    #110558

    Purpose

    miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    lacIq-Ptac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-puro LEF1

    Plasmid

    #27023

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6p-2-EWSR1

    Plasmid

    #46384

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS-N-TEV-Twin-Strep

    Plasmid

    #202526

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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