We narrowed to 2,677 results for: sfGFP
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Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
TagsHexahistidine tag and Superfolder GFPExpressionBacterialPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sender_plasmid1
Plasmid#140692PurposeEncodes constitutive yellow (sfYFP) fluorescence for identificationDepositorInsertsfYFP
TagsssrA degradation tag (ASV)ExpressionBacterialPromoterpIq constitutive promoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_004
Plasmid#123961PurposeEncodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertcRosa26 destination
ExpressionMammalianAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_028
Plasmid#123968PurposeEncodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertcRosa26 landing pad destination vector
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_045
Plasmid#123975PurposeEncodes the cROSA26 destination with hygyomycin resistance cassette as a type 0 part to be used in the MTK systemDepositorInsertcROSA26 DEST (hygro)
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_050
Plasmid#123918PurposeEncodes the saCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK systemDepositorInsertSaCas9-sgRNA-GFPDROPOUT
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_056
Plasmid#123921PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-hu6_20N_cr3
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_016
Plasmid#123931PurposeEncodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPhic31attB tagBFP destination vector
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_002
Plasmid#123900PurposeEncodes the spCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-GFPDROPOUT
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C92m
Plasmid#121000PurposeMoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).DepositorInsertsfCFPpdt3 (Chen,Bennett)
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C100m
Plasmid#121004PurposeMoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C101m
Plasmid#121005PurposeMoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).DepositorInsertsfCFP with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C20m
Plasmid#120961PurposeMoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)DepositorInsertsfYFP-ssrA (has BbsI site)
UseSynthetic BiologyTagsssrA degradation tagAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9FnCpf1GG
Plasmid#131013PurposeBackbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.DepositorInsertsdirect repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a
UseCRISPRExpressionBacterialAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only