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Plasmid#50895PurposeExpresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationS716C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)
Plasmid#50900PurposeExpresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationI721C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)
Plasmid#50908PurposeExpresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V W732C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)
Plasmid#50903PurposeExpresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC722S C723V I724C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)
Plasmid#50904PurposeExpresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V V726C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)
Plasmid#50898PurposeExpresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationG719C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSELIS-RamR
Plasmid#202631PurposeEnables dual growth and fluorescence based enrichment of RamR biosensorsDepositorInsertSh ble
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLambda promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW842_pCAG-pExpr-FP-DECODER
Plasmid#87551PurposeExpresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinasesDepositorInsertmtagBFP, EGFP, iRFP720, mRuby2
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRF
Plasmid#66936PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV-40Available sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRL1
Plasmid#153997PurposesgRNA in vitro transcription plasmid.DepositorTypeEmpty backboneUseCRISPR; Sgrna under a t7 promoter for in vitro tr…TagsExpressionMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIS-krPAM2
Plasmid#25457DepositorInsertPAM2 (Pam Rat)
UseTagsExpressionMammalianMutationExon A deleted (exon A extends from nt 1475 to n…PromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRR
Plasmid#66937PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV-40Available sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA
Plasmid#87584PurposePV5 for 3-input, 2-output Full AdderDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5089
Plasmid#85125PurposeKaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.DepositorInsertPkaiB-kaiB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW829_pCAG-pExpr-LUT
Plasmid#87554PurposeEncodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinasesDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#87560PurposeExpresses aa397-423 of Flp recombinase fused to PYL CIDDepositorInsertFlpO aa397-423
UseCre/Lox and Synthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#87559PurposeExpresses aa1-396 of Flp recombinase fused to ABI CIDDepositorInsertFlpO aa1-396
UseCre/Lox and Synthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-C. thermophilum LIC
Plasmid#74780Purposeencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilumDepositorInsertdynein light intermediate chain (Chaetomium thermophilum)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA
Plasmid#87587PurposePV8 for 3-input, 2-output Full AdderDepositorInsertEGFP, mCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYO411
Plasmid#235732PurposeProtein expression of ZZ-FL yeast Atg14 in yeastDepositorInsertATG14
UseTags3xTEV-ZZExpressionYeastMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO69
Plasmid#235747PurposeProtein expression of FL ScVPS34 in yeastDepositorInsertVPS34
UseTagsExpressionYeastMutationS2APromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO361
Plasmid#235748PurposeProtein expression of ScVPS34 HELCATDepositorInsertVPS34 HELCAT
UseTagsExpressionYeastMutationaa 268-875PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO227
Plasmid#235749PurposeProtein expression of ZZ-FL ScVPS30DepositorInsertVPS30
UseTagsZZ-3xTEVExpressionYeastMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO832
Plasmid#235755PurposeProtein expression of ScVPS30 (1-215E), untaggedDepositorInsertScVPS30
UseTagsExpressionYeastMutationaa 1-215EPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-M2
Plasmid#225735PurposeTo express M2 (219-399) in E. coliDepositorInsertmce3R
UseTags6xHisExpressionBacterialMutationaa 219-399PromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMV261-igr3K253E
Plasmid#225739PurposeTo express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in MycobacteriaDepositorInsertMyc-igr3K253E
UseTagsc-MycExpressionBacterialMutationigr3 K253EPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only