Addgene

We narrowed to 774 results for: golden gate assembly

Showing: 361 - 390 of 774 results

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pPOM012_P5_ConR11

Plasmid

#216437

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM011_P5_ConR10

Plasmid

#216436

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM010_P5_ConR9

Plasmid

#216435

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM009_P5_ConR8

Plasmid

#216434

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R8

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM008_P5_ConR7

Plasmid

#216433

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R7

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM007_P5_ConR6

Plasmid

#216432

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM006_P1_ConL11

Plasmid

#216431

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM005_P1_ConL10

Plasmid

#216430

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM004_P1_ConL9

Plasmid

#216429

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM003_P1_ConL8

Plasmid

#216428

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L8

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM002_P1_ConL7

Plasmid

#216427

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L7

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM001_P1_ConL6

Plasmid

#216426

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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C9m

Plasmid

#120958

Purpose

MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file

Depositor

Richard Murray

Insert

deCFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C76m

Plasmid

#120988

Purpose

MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

KanR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U4m

Plasmid

#121017

Purpose

MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

UTR1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

Plasmid

#195049

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pPGI

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLDP1

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFBA

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGPD

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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