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Showing: 241 - 260 of 546 results

  1. 2QO2 (EPHA3)

    Plasmid
    #101271
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPHA3 (EPHA3 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101271-plasmid-map-sequence-id-193505

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  2. pBP-HexHis

    Plasmid
    #72983
    Purpose
    Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Polyhistidine (HHHHHH) N-terminal tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72983-plasmid-map-sequence-id-133831

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  4. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pET28a-HsSENP7

    Plasmid
    #71470
    Purpose
    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid
    #176573
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176573-plasmid-map-sequence-id-348271

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  11. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176577
    Purpose
    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Hexahistidine tag and mVenus
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176577-plasmid-map-sequence-id-348269

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  17. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 546 results