Addgene

Showing: 241 - 260 of 560 results

Results per page:

Sort results by:

AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Article

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-LC3A WT

Plasmid

#123089

Purpose

Expresses 3xFLAG-LC3A wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-LC3C WT

Plasmid

#123095

Purpose

Expresses 3xFLAG-LC3C wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM60-FLAG-SLC38A9.1

Plasmid

#71858

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-SLC38A9.1

Plasmid

#71855

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-CMV-mC-G-LC3B-P

Plasmid

#124974

Purpose

evaluate autophagy

Depositor

Kazuhiro Oka

Article

Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

Insert

mCherry-GFP-LC3B

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV mKeima-LC3

Plasmid

#189006

Purpose

Fluorescent reporter for autophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

mKeima, LC3 (Map1lc3b Rat)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

Plasmid

#214977

Purpose

Flag tagged LDS mutant LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B_mut (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

LDS mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-LC3-YFP

Plasmid

#99571

Purpose

Lentiviral expression of YFP-tagged LC3

Depositor

Edward Campbell

Article

Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

Insert

LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Aquamarine-LC3B-TdLanYFP

Plasmid

#228560

Purpose

Encodes the LC3B biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

Aquamarine and TdLanYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABEpuro mTurquoise2 LC3B

Plasmid

#78518

Purpose

Fluorescent reporter for LC3B visualization

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

retroviral

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-GFP-hLC3WT

Plasmid

#24987

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV mScarlet-LC3B

Plasmid

#200431

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge