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Showing: 241 - 260 of 284 results

  1. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  3. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

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  4. pEP4 E02S EN2K

    Plasmid
    #20925
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20925-plasmid-map-sequence-id-9761

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  9. pEP4 E02S EN2L

    Plasmid
    #20922
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20922-plasmid-map-sequence-id-9758

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  16. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid
    #193294
    Purpose
    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193294-plasmid-map-sequence-id-379650

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  17. pCXLE-SOX2-17-p2a-KLF4

    Plasmid
    #193290
    Purpose
    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193290-plasmid-map-sequence-id-379665

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  19. pEP4 E02S EM2K

    Plasmid
    #20923
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20923-plasmid-map-sequence-id-272720

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Showing: 241 - 260 of 284 results