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We narrowed to 259 results for: T7 rna polymerase

Showing: 241 - 259 of 259 results

  1. pCFB8455

    Plasmid
    #175207
    Purpose
    Xenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
    Depositor
    Irina Borodina
    Article
    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.
    Insert
    YCR075C
    Use
    Mrna expression, injection into xenopus oocytes
    Tags
    PolyA
    Promoter
    T7
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    175207-plasmid-map-sequence-id-348319

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  6. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99214-plasmid-map-sequence-id-192369

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  7. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  8. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  10. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Promoter
    Pol II readthrough transcription after integration
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  12. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  13. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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  14. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  16. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  18. pPos-Ma-CmR-TAG111

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

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  19. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Expression
    Bacterial
    Available Since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 259 of 259 results