We narrowed to 9,340 results for: UTY

1. hFLN29 C60-end/pCMV-14-FLAG

   Plasmid

   #73709

   Purpose

   Express human TRAFD1 (FLN29) fromC60 to end in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   TRAFD1 (TRAFD1 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   deletion of first 59 aa from full lenght TRAFD1

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pK18msB

   Plasmid

   #177839

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

   Depositor

   Christopher Johnson

   Article

   Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pConRef-2H12.D11

   Plasmid

   #221151

   Purpose

   cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQD0KN0750

   Plasmid

   #185627

   Purpose

   Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   lacZ
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-DrFLINC-AKAR-Kras

   Plasmid

   #182334

   Purpose

   Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DrFLINC-AKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMP71-mIL15-IL12rb1-sushi-il12rb2

   Plasmid

   #174606

   Purpose

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXPA-fapO-RgTALsyn

   Plasmid

   #100946

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   GAP (Constitutive)

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQDKN0761

   Plasmid

   #185630

   Purpose

   Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD1CB0107

    Plasmid

    #177040

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD1CB0202

    Plasmid

    #185626

    Purpose

    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hPlekhm1-C/pIRES puro Glue (S778-A1056)

    Plasmid

    #73456

    Purpose

    Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. hPlekhm1-C/pIRES puro Glue (H987-end)

    Plasmid

    #73458

    Purpose

    Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. hPlekhm1-C/pIRES puro Glue (S778-Q986)

    Plasmid

    #73457

    Purpose

    Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

15. hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

    Plasmid

    #73455

    Purpose

    Express human middle part (Q513-E784) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pEPQDKN0760

    Plasmid

    #185629

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0KN0751

    Plasmid

    #185628

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD1CB0869

    Plasmid

    #177086

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQD1CB0867

    Plasmid

    #177085

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge