We narrowed to 922 results for: cas12a

1. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

   Plasmid

   #210604

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional activator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   hyperdCas12a-miniVPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

   Plasmid

   #114069

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

   Plasmid

   #140197

   Purpose

   Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

   Plasmid

   #210605

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional base editor

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   ABE8e-hyperdCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

   Plasmid

   #140193

   Purpose

   Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dLbCas12a-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNAse deactivated

   Promoter

   PgpdA and PtrpC

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

   Plasmid

   #236386

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D156R, D235R, E292R, D350R

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

   Plasmid

   #114078

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

   Plasmid

   #195543

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

   Plasmid

   #114094

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (S542R/K607R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R and K607R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid

    #202042

    Purpose

    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    ddCas12a(E993A)-1xNLS-[Activ]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid

    #202043

    Purpose

    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    ddCas12a(E993A)-3xNLS-[Activ]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

    Plasmid

    #202044

    Purpose

    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    ddCas12a(E993A)-3xNLS-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236376

    Purpose

    Overexpression of HyperdFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E184R, T279R, D340R, D409R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

    Plasmid

    #236368

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236375

    Purpose

    Overexpression of dFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236363

    Purpose

    Overexpression of endAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    endAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236362

    Purpose

    Overexpression of HyperdAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, D256R, E321R, E371R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236361

    Purpose

    Overexpression of dAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge