We narrowed to 2,596 results for: ubiquitin

1. pGAD424-Rabex-5

   Plasmid

   #197043

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   Rabex-5 (RABGEF1 Bovine)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Duet1-6xHis-TEV-OPTN

   Plasmid

   #190192

   Purpose

   Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet1-His-TEV-OTC_d1_Ub

   Plasmid

   #170729

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   The C-terminus of OTC was truncated by removing o…

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 Ub-NLRP1 L1215

   Plasmid

   #166828

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 Ub-NLRP1 S1213

   Plasmid

   #166827

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221 Ub-NLRP1 L1218

   Plasmid

   #166829

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6p1 AO7R

   Plasmid

   #137842

   Purpose

   GST tagged AO7 encoding aa 126-216 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-HA-RNF68 [Y252D]

   Plasmid

   #126550

   Purpose

   Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF68 (PCGF1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Y252D point mutant

   Promoter

   LTR

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-BirA-UBE3B-Puro

   Plasmid

   #107351

   Purpose

   Expresses UBE3B fused to BirA in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   BirA-UBE3B (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   Biotin ligase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCMV-FLAG-WRAP53beta-siRNA-resistant

    Plasmid

    #64680

    Purpose

    expresses siRNA resistant WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    CMV

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only