We narrowed to 15,050 results for: pET

1. pET SUMO-syt7 (136-403)

   Plasmid

   #213621

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET SUMO-Doc2β C2AB (G361C)

   Plasmid

   #213631

   Purpose

   Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   Doc2β C2AB (G361C) (Doc2b Rat)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   G361C, all original cysteines mutated to alanines

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET SUMO-cPLA2 C2 domain (Y96C)

   Plasmid

   #213633

   Purpose

   Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   cPLA2 C2 domain (Y96C) (PLA2G4A Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Y96C, C139A, C141S

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

   Plasmid

   #214339

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (Il18 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ENGD29DDEN_N36Y_H41E

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-His6-CUbo(K63R)-MCS

   Plasmid

   #212727

   Purpose

   Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-His6-CUbo(K48R)-MCS

   Plasmid

   #212726

   Purpose

   Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-His6-CUbo(K63R)-GFP

   Plasmid

   #212722

   Purpose

   Bacterial expression of His6-CUbo(K63R)-GFP

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   GFP
   

   Tags

   6xHis-CUbo(K63R)

   Expression

   Bacterial

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-His6-CUbo(K48R)-GFP

   Plasmid

   #212721

   Purpose

   Bacterial expression of His6-CUbo(K48R)-GFP

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   GFP
   

   Tags

   6xHis-CUbo(K48R)

   Expression

   Bacterial

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

   Plasmid

   #208630

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   Δ1~34,Δ215~284

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208632

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 53~77, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208624

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~77, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDUET-GST AO7RE ∆238-245

    Plasmid

    #139318

    Purpose

    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+His SUMO Δ37 PPM1H

    Plasmid

    #207334

    Purpose

    Bacterial expression of His SUMO Δ37 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Δ37

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+His SUMO Rab10(Q68L)

    Plasmid

    #207342

    Purpose

    Bacterial expression of His SUMO Rab10(Q68L)

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    Rab10 (RAB10 Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Q68L

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30a- phage MazG-like/Atd1

    Plasmid

    #195244

    Purpose

    Expersses phage MazG-like/Atd1

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    phage MazG
    

    Tags

    Flag and TwinStrep

    Expression

    Bacterial

    Mutation

    Catalytic site mutant

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-20b_HtrA2(delta(133))_S306A_6His

    Plasmid

    #197975

    Purpose

    E. coli expression of HtrA2(delta133)-6His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    delta(1-133), S306A

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM-60-NcQDE2 _506-938-A602E_M

    Plasmid

    #146964

    Purpose

    Bacterial Expression of NcQde-2_506-938-A602E

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-A602E
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM-60-NcQDE2 _506-938-H609W_M

    Plasmid

    #146965

    Purpose

    Bacterial Expression of NcQde-2_506-938-H609W

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-H609W
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM-60-NcQDE2 _506-938-K634A_M

    Plasmid

    #146966

    Purpose

    Bacterial Expression of NcQde-2_506-938-K634A

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-K634A
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETM-60-NcQDE2 _506-938-R895A_M

    Plasmid

    #146967

    Purpose

    Bacterial Expression of NcQde-2_506-938-R895A

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-R895A
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETM-60-NcQDE2 _506-938-Y595L_M

    Plasmid

    #146968

    Purpose

    Bacterial Expression of NcQde-2_506-938-Y595L

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-Y595L
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge