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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,237 results for: FIE

Showing: 5161 - 5200 of 8237 results

  11. pHT-497

    Plasmid
    #87756
    Purpose
    Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)
    Depositor
    Yu-Wen Hwang
    Insert
    Gene coding for rat DYRK1A kinase domain (residues 1-497)
    Use
    Tags
    6X histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87756-plasmid-map-sequence-id-173678

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  12. pLXIN2-EGFP

    Plasmid
    #99204
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    EGFP
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was inserted 5' to ATG to …
    Promoter
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    99204-plasmid-map-sequence-id-192204

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  16. Vimentin/pBSSK+

    Plasmid
    #98133
    Purpose
    Vimentin (NM_003380) in pBSSK+
    Depositor
    Rita Shiang
    Insert
    Vimentin (VIM Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98133-plasmid-map-sequence-id-189654

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  24. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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  25. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  26. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  27. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

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  28. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203977-plasmid-map-sequence-id-403301

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  29. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

    Plasmid
    #198022
    Purpose
    LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population
    Depositor
    Donald Kohn
    Insert
    CD3D c.202C>T-cDNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198022-plasmid-map-sequence-id-390932

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  30. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid
    #183704
    Purpose
    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM4D(Gi)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183704-plasmid-map-sequence-id-360830

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  31. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid
    #183705
    Purpose
    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM3D(Gq)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183705-plasmid-map-sequence-id-360768

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  33. psicheck_UBE2V1_3UTR

    Plasmid
    #65885
    Purpose
    Luciferase assay vector, assessing regulation of UBE2V1 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    UBE2V1 (UBE2V1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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Showing: 5161 - 5200 of 8237 results