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pAcBac1-Pyl-TAA

Plasmid

#228783

Purpose

Pyl aaRS/tRNA system for mammalian TAA suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Expression

Mammalian

Mutation

TAA suppressor

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pUt-mNF-Cas13d

Plasmid

#224789

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr1-gRNA-EGFP

Plasmid

#226000

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr2-gRNA-EGFP

Plasmid

#226001

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA1-EGFP

Plasmid

#226004

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA2-EGFP

Plasmid

#226005

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AWLDLE tail 361-1749

Plasmid

#224412

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Tags

FRB-3myc

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AS1371A tail 361-1749

Plasmid

#224411

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Tags

FRB-3myc

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLeo-A-GEMS(JAK-STAT)

Plasmid

#209109

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a3-GEMS(JAK-STAT)

Plasmid

#209111

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a3-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-B-a2-GEMS(JAK-STAT)

Plasmid

#209112

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

B-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-G-a2-GEMS(JAK-STAT)

Plasmid

#209113

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

G-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BR1-P2A-FusionRed

Plasmid

#192819

Purpose

Expresses BR1 with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148410

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1-1084-shRNAres_AC

Plasmid

#148411

Purpose

Mammalian Expression of HsNot1iso1_1-1084-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1-1084-shRNAres (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1085-1317-shRNAres_AC

Plasmid

#148412

Purpose

Mammalian Expression of HsNot1iso1_1085-1317-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1085-1317-shRNAres (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1085-1605_AC

Plasmid

#148413

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1606-1840-shRNAres_AC

Plasmid

#148415

Purpose

Mammalian Expression of HsNot1iso1_1606-1840-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1606-1840-shRNAres (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1841-2376-shRNAres_AC

Plasmid

#148416

Purpose

Mammalian Expression of HsNot1iso1_1841-2376-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1841-2376-shRNAres (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_1-295_AC

Plasmid

#148418

Purpose

Mammalian Expression of HsRck_1-295

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_1-295 (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-QHTRtoAAAA_AC

Plasmid

#148421

Purpose

Mammalian Expression of HsRck_296-472-QHTRtoAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-QHTRtoAAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-RKKtoAAA_AC

Plasmid

#148422

Purpose

Mammalian Expression of HsRck_296-472-RKKtoAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-RKKtoAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-RQNtoAAA_AC

Plasmid

#148423

Purpose

Mammalian Expression of HsRck_296-472-RQNtoAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-RQNtoAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148429

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-E236Q_H

Plasmid

#146456

Purpose

Mammalian Expression of HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-E236Q (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

Plasmid

#196112

Purpose

mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196111

Purpose

mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP2-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

Plasmid

#196109

Purpose

mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zTagRFP658-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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