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Plasmid#222393PurposeExpress in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
UseLentiviralTagsEGFP and Human UTROPHIN 1-261ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26737-G…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRH337: AAV-hsyn-CheRiff-eGFP
Plasmid#51697PurposeAAV production of CheRiff channelrhodopsin actuatorDepositorInsertCheRiff-eGFP
UseAAVTagseGFPExpressionMutationPromoterhSynapsinAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-EGFP-T2A-Bsd
Plasmid#193198PurposeLentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance markerDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(D10A)-T2A-EGFP
Plasmid#221230PurposeExpress nCas9 nickase (D10) with GFP reporterDepositorInsertSpCas9(D10A)-T2A-EGFP
UseTagsExpressionMammalianMutationD10APromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-EGFP (RTW4177)
Plasmid#139988PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Blast (w818-1)
Plasmid#27568DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-B-Seipin-miniIAA7-mEGFP
Plasmid#129719PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mEGFPExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-DiO-eGFP-2A-mCherry_D4H
Plasmid#194880PurposeRatiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-D4H
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ODD-EGFP-IRES-dTomato
Plasmid#87218Purposelentiviral expression of ODD-EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationODD domainPromoterEF1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A2-Neo
Plasmid#189743PurposeThe construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A2 (ALDH1A2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvpu/env
Plasmid#101345PurposeDeletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RREDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vpu/EnvPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII-ShadowG
Plasmid#167475PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-tmpknot-epegRNA
Plasmid#214092PurposeLentiviral vector expressing epegRNA to induce EGFR L858R mutationDepositorInsertEGFR L858R epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-2A-TetanusToxin
Plasmid#166603PurposeEncodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TREDepositorInsertEGFP-2A-Tetanus Toxin
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG
Plasmid#162086PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-HA-FLAG
UseLentiviralTagsVSVg-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1
Plasmid#162093PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-HA-AU1
UseLentiviralTagsStrepTagII-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1
Plasmid#162096PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-HA-AU1
UseLentiviralTagsProtC-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA
Plasmid#162083PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-ProtC-HA
UseLentiviralTagsVSVg-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-eDHFR(69K6)-MCS
Plasmid#172100PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)-20aa
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterHSPA7 promoter and PGK promoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-WT-Full-length
Plasmid#194548PurposeMammalian Expression of mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL-U6-sgRNA-SFFV-Puro-P2A-EGFP
Plasmid#175037PurposeLentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.DepositorInsertSp sgRNA scaffold
UseLentiviralTagsExpressionMutationPromoterU6Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-CMV-DsRed-IRES-d1EGFP
Plasmid#41942PurposeRetroviral expression of DsRed in mammalian cellsDepositorInsertDsRed
UseRetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCX-EGFP beta5 integrin receptor
Plasmid#14996DepositorInsertbeta5 integrin receptor (ITGB5 Human)
UseTagsIRES EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb-IRES-eGFP-W
Plasmid#32519DepositorInsertRheb (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W
Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(CTRL)-CMV-eGFP
Plasmid#194017PurposeExpresses a gRNA that targets the LacZ gene (serves as control) and eGFPDepositorInsertssgRNA(CTRL)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(H840A)-T2A-EGFP
Plasmid#221231PurposeExpress nCas9 nickase (H840A) with GFP reporterDepositorInsertSpCas9(H840A)-T2A-EGFP
UseTagsExpressionMammalianMutationH840APromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvif/vpr
Plasmid#101344PurposeDeletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequenceDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vif/VprPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight eGFP Puro (w771-1)
Plasmid#26431Purpose2nd generation lentiviral vectorDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral; Tet-on advanced vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEN716 - pCAGGS-eGFP-3Xnls-bpA
Plasmid#156435PurposeFor transient expression of nuclear eGFP in mammalian cellsDepositorInserteGFP
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1 F280E
Plasmid#203801PurposeAAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorInsertACAP1 (ACAP1 Human)
UseAAVTagseGFPExpressionMammalianMutationF280EPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPExpressionMutationPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-gfap(Intron1/5'/Exon1-zebrafish)
Plasmid#39761DepositorInsertzebrafish gfap promoter- EGFP (gfap Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromotergfapAvailable sinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
IC65 pEGFPN1 anti-IRSp53 nanobody
Plasmid#135227PurposeMammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only