Addgene

We narrowed to 10,225 results for: T7

Showing: 5201 - 5240 of 10225 results

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pVF30

Plasmid

#229472

Purpose

Expresses RssB with R115A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (R115A)

Use

Tags

Expression

Bacterial

Mutation

R115A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF29

Plasmid

#229473

Purpose

Expresses RssB with N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (N256A)

Use

Tags

Expression

Bacterial

Mutation

N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF28

Plasmid

#229469

Purpose

Expresses RssB with D104A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Use

Tags

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF31

Plasmid

#229468

Purpose

Expresses RssB with Q254A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A)

Use

Tags

Expression

Bacterial

Mutation

Q254A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF16

Plasmid

#229465

Purpose

Expresses RssB with D58P mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D58P)

Use

Tags

Expression

Bacterial

Mutation

D58P

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS11

Plasmid

#229464

Purpose

Expresses RssB with His Tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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K560-Halo-Strep

Plasmid

#219681

Purpose

Mammalian expression of KIF5B(1-560)-HaloTag-Strep

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Use

Tags

HaloTag, Strep tag

Expression

Mammalian

Mutation

1-560

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200791

Purpose

Mammalian expression of KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5B-SNAPf-HisTag-FLAG

Plasmid

#200799

Purpose

Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Use

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Article

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Use

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V1

Plasmid

#226538

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V2

Plasmid

#226539

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V3

Plasmid

#226540

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X5

Plasmid

#226547

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X5

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X7

Plasmid

#226549

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X7

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X7

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X1

Plasmid

#226543

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V5

Plasmid

#226542

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V5

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X4

Plasmid

#226546

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swapX3

Plasmid

#226545

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V4

Plasmid

#226541

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM420 GFP-titin-ssrA substrate

Plasmid

#226362

Purpose

Expression of GFP-titin-ssrA substrate for 26S proteasome

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

GFP-titin substrate

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSRK51

Plasmid

#223722

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

Sreejith Raran-Kurussi

Article

Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

Insert

TEV protease

Use

Tags

NT* (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

T7

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP580

Plasmid

#222959

Purpose

Expresses R141S K142S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine and Lysine 142 to …

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP579

Plasmid

#222958

Purpose

Expresses R36S K51S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine and Lysine 51 to Se…

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1478

Plasmid

#213804

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1476

Plasmid

#213802

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GST-ATG13 (363-517)

Plasmid

#222823

Purpose

Bacteria expression of His-GST -ATG13 C-terminal

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1477

Plasmid

#213803

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5201 - 5240 of 10225 results

Results per page:

Sort results by: