We narrowed to 12,370 results for: NUC

1. DS-ST1casN-

   Plasmid

   #48659

   Purpose

   Bacterial nuclease-null ST1 Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   ST1 tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2xEGFP-N1

   Plasmid

   #122162

   Purpose

   Expression of 2xEGFP

   Depositor

   Georg Mayr

   Article

   Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

   Insert

   2xEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCWU6-xylR-E-lepB

   Plasmid

   #190725

   Purpose

   fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-lepB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTagRFP-PCNA-19-SV40NLS-4

   Plasmid

   #57994

   Purpose

   Localization: Nucleus, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pFLlR5

   Plasmid

   #149481

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LacI-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLlacO-1

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFVR5

   Plasmid

   #149489

   Purpose

   Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   VanR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PVanCC

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCyR5

   Plasmid

   #149480

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3s-Cas9-HN

   Plasmid

   #104171

   Purpose

   Cas9-WT mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   Cas9-WT
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPcR5

   Plasmid

   #149484

   Purpose

   Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   PcaU(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3B5B

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PL-452 C-Flag-4C

    Plasmid

    #19180

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

    Insert

    FLAG tetracysteine tag
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pT7goldRNA

    Plasmid

    #47930

    Purpose

    In vitro transcription of golden gRNA

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    golden gRNA (slc24a5 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKER-mRuby2

    Plasmid

    #85977

    Purpose

    Expresses mRuby2 from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

    Plasmid

    #196831

    Purpose

    Express PUFc-RESCUE-S

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    PUFc-RESCUE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. peft-3::cas-9::tbb-2 3'UTR

    Plasmid

    #48960

    Purpose

    this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. p8xEGFP-N1

    Plasmid

    #122168

    Purpose

    Expression of 8xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    8xEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUPER retro puro macroH2A1 shRNA

    Plasmid

    #30517

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    mH2A1 shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMLB10

    Plasmid

    #177273

    Purpose

    GAL1p:T7RNAP(F11L;T613A):CYC1t

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Insert

    T7RNAP(F11L;T613A)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    F11L T613A

    Promoter

    GAL1

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFCiK5

    Plasmid

    #167514

    Purpose

    Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinRAM-mKelly2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP8A-ccdB

    Plasmid

    #83883

    Purpose

    Destination vector for integration at HIS3, expression from the ADH1 promoter

    Depositor

    Jennifer Nemhauser

    Article

    Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-Map7emtb

    Plasmid

    #46078

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7emtb
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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