We narrowed to 13,271 results for: cDNA

1. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

   Plasmid

   #129513

   Purpose

   Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 C88S (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-GoldenGate-VP64

   Plasmid

   #47389

   Purpose

   Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   FLAG, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Nrxn1beta AS4(+)-Fc

   Plasmid

   #59314

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele, Tito Serafini

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
   

   Use

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 tdAPX W41F

   Plasmid

   #40863

   Purpose

   Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   tandem dimer pea APX W41F
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W41F mutation

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. GABPA_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176970

   Purpose

   Mammalian expression vector encoding GABPA and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GABPA (GABPA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mDIS3-FLAG

   Plasmid

   #60046

   Purpose

   Mammalian expression of FLAG-tagged mouse DIS3

   Depositor

   Zissimos Mourelatos

   Article

   Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

   Insert

   DIS3 (Dis3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-FRT-TO-FH-eGFP

   Plasmid

   #157734

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-eGFP
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. α1GA-HE3-pcDNA3

   Plasmid

   #45812

   Depositor

   Edward Perez-Reyes

   Insert

   α1Ga (Cacna1g Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Mcm5-mCer

   Plasmid

   #211393

   Purpose

   mammalian expression of human Mcm5 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm5 (MCM5 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-IFT57-pcDNA3

    Plasmid

    #47326

    Purpose

    Expresses FLAG epitope tagged IFT57 in mammalian cells

    Depositor

    Richard Maurer

    Article

    Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

    Insert

    IFT57 (Ift57 Mouse)
    

    Use

    Tags

    FLAG epitope

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4TO-24xGCN4_v4-kif18b

    Plasmid

    #74934

    Purpose

    Translation reporter that lacks PP7 sites in the 3'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-ccdB-TwinStrep

    Plasmid

    #214988

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-Nsp9

    Plasmid

    #157703

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.2/NS5-YFP

    Plasmid

    #120831

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP
    

    Use

    Tags

    eYFP-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AKAP79-Dronpa

    Plasmid

    #182332

    Purpose

    AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    AKAP79-Dronpa (AKAP5 Human)
    

    Use

    Tags

    Full-length AKAP79

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ShhN-Ren

    Plasmid

    #37677

    Depositor

    Philip Beachy

    Article

    Ma et al Cell. 2002 Oct 4;111(1):63-75.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    soluble form (see comments)

    Promoter

    CMV

    Available Since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA flag-Msi1

    Plasmid

    #184029

    Purpose

    Msi1 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Msi1 (Msi1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MAP3K5 (R256C)

    Plasmid

    #47105

    Purpose

    Expresses mutant (R256C) human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 256 to cysteine

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only