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Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Danio alpha Bb-crystallin 1kb promoter/AcGFP

Plasmid

#98098

Purpose

aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio aBb-crystallin 1 kb fragment (-1074/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA

Plasmid

#108831

Purpose

Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MI-Luciferase

Plasmid

#75018

Purpose

Encodes the expression of Luciferase (with out any fluorescent reporter)

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2015 May 7;125(19):2968-73. doi:

Insert

firefly Luciferase

Use

Luciferase and Retroviral

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

Plasmid

#154779

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA

Plasmid

#108765

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108766

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

Plasmid

#154770

Purpose

plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, G1 PylT with A41AA and C5…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCINeo-hFX(I154F)-HA

Plasmid

#14977

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Mutation

changed isoleucine 154 to phenylalanine

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
KHBD00517

Plasmid

#39618

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5893 (D Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108832

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1920

Plasmid

#82562

Purpose

High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple253

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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KHBD00273

Plasmid

#34401

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11987 (tgo Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00167

Plasmid

#34320

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12399 (Mad Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00506

Plasmid

#39609

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12196 (egg Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-2B-Nkx2-5 delta HD-G8

Plasmid

#98614

Purpose

Gateway entry vector NKX2-5 _HD

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 _HD (Nkx2-5 Mouse)

Use

Gateway Cloning

Mutation

Deletion of the homeodomain. Replaced by 8 glycin…

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1941

Plasmid

#82564

Purpose

High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple264

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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KHBD00510

Plasmid

#39611

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8365 (E(spl)m8-HLH Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00458

Plasmid

#39571

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10037 (vvl Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only