We narrowed to 9,259 results for: pho

1. myc-Atg14 S29A

   Plasmid

   #86756

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S29A

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCin4-3xFLAG-MKL1 S454A

   Plasmid

   #19844

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S454A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 454 to Alanine

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

5. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Use

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Y587E EphA2 pcDNA3

   Plasmid

   #102730

   Purpose

   Expression of EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

   Insert

   EphA2 (Epha2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y587E (TAT > GAG)

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Fes-SH2

   Plasmid

   #46426

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Fes (FES Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 396-497

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV HA hRB delta CDK + S780

   Plasmid

   #58915

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S780 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-4T Doc2beta C2AB(125) G361C

   Plasmid

   #134694

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G361C, C145A, C217A, C249A, C290A, C338A, C387A

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Braf R188L

    Plasmid

    #118330

    Purpose

    Expression of human Braf

    Depositor

    Philip Stork

    Article

    Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

    Insert

    Braf R188L (BRAF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R188L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX p85a(N)-SH2

    Plasmid

    #46464

    Depositor

    Bruce Mayer, Leszek Kotula

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(N) (PIK3R1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 321-434

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pClneoMyc MOB1 T12/35A

    Plasmid

    #47938

    Purpose

    expresses Myc tagged human MOB1 containing T12A and T35A mutations

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A and T35A

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX SHP-1(N)-SH2

    Plasmid

    #46495

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(N) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-109

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-C3-PLK4-S305E-3xFLAG

    Plasmid

    #69840

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S305E

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GB1-PRD(703-868, P801G)-Strep

    Plasmid

    #164444

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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