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C232-E25: attB2r-mVenus-attB3

Plasmid

#162905

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E34: attB2r-iRFP670-attB3

Plasmid

#162908

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E19: attB4-mPol2p>-attB1r

Plasmid

#162971

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E15: attB5r-mVenus-nostop-attB1r

Plasmid

#162943

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E26: attB4-SV40p>-attB1r

Plasmid

#162923

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 immediate early promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E07: attB2r-HA-attB3

Plasmid

#162911

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E06: attB2r-FLAG-attB3

Plasmid

#162910

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E05: attB2r-V5-attB3

Plasmid

#162909

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnma1b

Plasmid

#164952

Purpose

Zebrafish kcnma1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1b (kcnma1b Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnmb2a

Plasmid

#164953

Purpose

Zebrafish kcnmb2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnmb2a (kcnmb2 Zebrafish)

Use

Pcr cloning vectror

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnmb2b

Plasmid

#164954

Purpose

Zebrafish kcnmb2b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnmb2b (si:ch211-247n2.1 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnmb3

Plasmid

#164955

Purpose

Zebrafish kcnmb3 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnmb3 (si:ch211-38m6.7 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnt1a

Plasmid

#164961

Purpose

Zebrafish kcnt1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt1a (kcnt1 Zebrafish)

Use

Gateway entry vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt1b

Plasmid

#164962

Purpose

Zebrafish kcnt1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt1b (si:dkey-21e5.1 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-23b

Plasmid

#112538

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-23b (MIR23B Human)

Use

Adenoviral

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-charge+

Plasmid

#160531

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336K, L340K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock

Plasmid

#160530

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-N342W

Plasmid

#160529

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-L340W

Plasmid

#160528

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-N338W

Plasmid

#160527

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-Y336W

Plasmid

#160526

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-no charge

Plasmid

#160525

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, E337A, N338A, K341A, N342A

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-charge+

Plasmid

#160524

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock

Plasmid

#160523

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-charge+

Plasmid

#160522

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-N342C

Plasmid

#160521

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-N342C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, N342C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-L340C

Plasmid

#160520

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-L340C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, L340C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-no charge

Plasmid

#160514

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

E337A, N338A, K341A, N342A

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-charge+

Plasmid

#160513

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UBQ10:sXVE:S11-GFP1–9

Plasmid

#125665

Purpose

Binary vector for the inducible expression of S11-GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

S11-GFP1–9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFD-NIa-Protease-TVMV

Plasmid

#137109

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

NIa Protease TVMV

Use

Synthetic Biology

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Plasmid

#137117

Purpose

Protease Switch Clone ID: 2-A5

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-EMTB-GFP-ePDZ

Plasmid

#135600

Purpose

Expression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

EMTB-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-Lifeact-GFP-ePDZ

Plasmid

#135602

Purpose

Expression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Promoter

Unknown

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCuHA-ATG13[3SD](426)

Plasmid

#122077

Purpose

Expressing HA-ATG13[3SD]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ATG13[3SD](315)

Plasmid

#122074

Purpose

Expressing GFP-Atg13[3SD]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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