We narrowed to 17,638 results for: URE

Showing: 2621 - 2640 of 17638 results

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pVeBtA2d7

Plasmid

#238989

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 174, 176, 177, 181-184

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA_F164R238

Plasmid

#216230

Purpose

S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed arginine 238 to serine, phenylalanine 168…

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_A467

Plasmid

#216234

Purpose

S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed alanine 467 to valine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_T522

Plasmid

#216235

Purpose

S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed threonine 522 to isoleucine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-Ark-L2

Plasmid

#186362

Purpose

Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Ciona robusta Aurora kinase A

Use

Expression of a fluorescent centrosome marker

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-HistoneH2B-L2

Plasmid

#186357

Purpose

Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Histone H2B (H2BC11 Human)

Use

Expression of a tagged nuclear marker

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

Plasmid

#186413

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli)

Use

Gateway destination vector

Tags

Nuclear localization signal

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_SpDHN1 (pBS0799)

Plasmid

#185261

Purpose

For the mammalian expression of the grass (Stipa purpurea) protein DHN_SpDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_SpDHN1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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L2_NOP1gRNA-Cas9-CsA

Plasmid

#136139

Purpose

Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_Cas9-NLS

Plasmid

#136122

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_AtcoCas9-NLS(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32ac-Flag

Plasmid

#107431

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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jam2a-pCRII

Plasmid

#68804

Purpose

For in situ antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Insert

jam2a (jam2a Zebrafish)

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
GoldyTALEN-moesina TAL1

Plasmid

#41831

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

moesin a (msna Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-moesina TAL2

Plasmid

#41832

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

moesin a (msna Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD4_3

Plasmid

#36376

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD4 (MBD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD4_2

Plasmid

#36375

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD4 (MBD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pT7II-2N4Rtau

Plasmid

#177653

Purpose

Recombinant protein synthesis of human Tau

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

Tau (2N4R) (MAPT Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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