We narrowed to 142,843 results for: ins

1. pcDNA3.1 EWS-myc-HIS

   Plasmid

   #46386

   Purpose

   expresses myc, HIS tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pJW2172

   Plasmid

   #163096

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-XRCC1

   Plasmid

   #215142

   Purpose

   Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJW2086

   Plasmid

   #154335

   Purpose

   Crispr repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCerulean-PCNA-HygroR

   Plasmid

   #215064

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMVA2

   Plasmid

   #119951

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A110G

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. YA1532: pAAV_ CAG-FLEX-paQuasAr3

   Plasmid

   #107702

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   paQuasAr3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Flag-Tet2 CD Mut

   Plasmid

   #72220

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pAWG-dCas9-VPR

   Plasmid

   #63802

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Insect

   Mutation

   Promoter

   act5c

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX0393

    Plasmid

    #205984

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM19

    Plasmid

    #123655

    Purpose

    Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pIEx

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabepuro3-p18 A61D

    Plasmid

    #24937

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A61D

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

14. YA1531: pAAV_CAG-FLEX-QuasAr3

    Plasmid

    #107701

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-FKBP-STING(pLxIS)

    Plasmid

    #221333

    Purpose

    Overexpression of STING CTT in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMS-eGFP-RTP801

    Plasmid

    #65057

    Purpose

    Expression of RTP801 in mammalian cells

    Depositor

    Cristina Malagelada, Lloyd Greene

    Article

    Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

    Insert

    DDIT4 (Ddit4 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Venus-Bcl2-pEGFP-C1

    Plasmid

    #177417

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCerulean-PCNA-PuroR

    Plasmid

    #215063

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge