We narrowed to 6,501 results for: nls
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Plasmid#261134PurposeFor C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertelt-2p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
ExpressionWormPromoterelt-2 minimal promoterAvailable sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM70
Plasmid#261135PurposeFor N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertelt-2p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
ExpressionWormPromoterelt-2 minimal promoterAvailable sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM34
Plasmid#261121PurposeFor C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertrgef-1p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
ExpressionWormPromoterrgef-1Available sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-MIOX
Plasmid#166680PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1948
Plasmid#154340PurposeEnhancer reporterDepositorInsertpes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
ExpressionWormMutationSilent mutations to remove piRNA sitesAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225246PurposeMammalian expression of iPASnFR-L in the nucleusDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiPASnFR-L
Tags3xFLAG and mScarlet3ExpressionMammalianAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTAL-MajSat-FLAG-NLS-VP64
Plasmid#69074PurposeExpresses TALE for mouse major satellite with VP64 and FLAG under CAG promoterDepositorInsertTALE
ExpressionMammalianAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-PGK-Puro
Plasmid#110844PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-2xNLS-mTurquoise2
Plasmid#104053PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoterDepositorInsert2xNLS-mTurquoise2
UseAAVTagsNLSPromoterGFAPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
Plasmid#222993PurposeMammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES mYFP
Plasmid#130712PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-F98-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
RANLS-IETD-BNES
Plasmid#60972PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACBEmax
Plasmid#139106PurposeMammalian ACBEmax expression vector (pSI-632)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP1594_2x_NLS
Plasmid#110625PurposeMammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSt1Cas9 LMD-9
None
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLBS.EF NLS-pHlu
Plasmid#129324PurposepH measurement, nucleus, lentivirusDepositorPublicationInsertpHluorin (from H. Imamura)
UseLentiviralAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP4 coiled-coil fused to the ABI domain
TagsMycExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only