We narrowed to 3,194 results for: pEGFP

1. SiriusGFP

   Plasmid

   #123201

   Purpose

   We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

   Insert

   SiriusGFP
   

   Use

   Mouse Targeting

   Tags

   N/A

   Expression

   Bacterial and Mammalian

   Mutation

   eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ITPKA-mTurquoise2

   Plasmid

   #137811

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA (ITPKA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hela kinase-dead MLCK-GFP

   Plasmid

   #46317

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase-dead (MYLK Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   E1626K

   Promoter

   CMV

   Available Since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. FKBP-beta2(616-951)-GFP

   Plasmid

   #100726

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available Since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-EGFP-C1-Flag-mFmr1(A)

   Plasmid

   #87913

   Purpose

   Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

   Depositor

   Stephanie Ceman

   Article

   Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   S499A

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. G-GECO1-Orai1

   Plasmid

   #73561

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   NM_032790.3 (ORAI1 Human, Synthetic)
   

   Tags

   G-GECO1

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PH-Btk(R28C)-GFP

   Plasmid

   #51464

   Purpose

   Non-PIP3 binding control for PH-Btk-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R28C

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pmCherry-Zdk1-EB1C

   Plasmid

   #107695

   Purpose

   C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Tags

   Zdk1 and mCherry

   Expression

   Mammalian

   Mutation

   EB1 aa 186-268

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fz4-GFP

   Plasmid

   #42197

   Depositor

   Robert Lefkowitz, Jeremy Nathans

   Article

   Chen et al Science. 2003 Sep 5;301(5638):1391-4.

   Insert

   Frizzled 4 (Fzd4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. SSpB-HaloTag-p63DH

    Plasmid

    #176116

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH2R-mCherry-N1

    Plasmid

    #84326

    Purpose

    Histamine-2 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H2R (HRH2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRY2clust-mCherry-RANK

    Plasmid

    #208612

    Purpose

    Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

    Depositor

    Tomohiro Ishii, Takao Nakata

    Article

    Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

    Insert

    CRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-beta1(617-949)-GFP

    Plasmid

    #100732

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP1B1 (AP1B1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid

    #172101

    Purpose

    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    22aa-eDHFR(69K6)-EGFP
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSpB-mCherry-p63DH

    Plasmid

    #176112

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VPS13C^halo∆1235-1748

    Plasmid

    #187297

    Purpose

    Express VPS13C truncation mutant in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS13C truncation mutant (VPS13C Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173862

    Purpose

    Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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