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Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVQ
Plasmid#200187PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editingDepositorInsertLbCas12a-RVQ
UseCRISPR; Gateway compatible lbcas12a-rv entry cloneTagsNLSExpressionPlantMutationG146R, R182V, E795QPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(C358S)
Plasmid#42513DepositorInsertPyruvate Kinase M2 Cys358->Ser
UseRetroviralTagsFlagExpressionMutationChanged Cysteine 358 to SerinePromoterCMVAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
p221k-erTq2CFP
Plasmid#71264PurposeEntry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertTq2CFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA S31N
Plasmid#19718DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationS31N mutant for dominant neg function. NOTE: lo…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA1
Plasmid#158479PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationSTOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_F(ab')2
Plasmid#105860PurposeExpression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertIgG3 F(ab')2 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEND-321_Cacnes_ori
Plasmid#225603PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIN1 (gpdA-cTerm-mGL-ptrA)
Plasmid#188735PurposeExpresses mGreenlantern for C-terminal taggingDepositorInsertgdpA-mGreenlantern PtrA
UseFilamentous fungal expressionTagsmGreenlanternExpressionMutationPromotergpdAAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIN2 (gpdA-nTerm-mGL-ptrA)
Plasmid#188736PurposeExpresses mGreenlantern for N-terminal taggingDepositorInsertgdpA-mGreenlantern PtrA
UseFilamentous fungal expressionTagsmGreenlanternExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN378 - pTRV2-CrGLK
Plasmid#203888PurposeViral silencing plasmid targeting the CrGLK gene from C. roseus.DepositorInsertCrGLK fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R
Plasmid#188542PurposeGateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editingDepositorInsertLbCas12a-D156R
UseCRISPR; Gateway compatible lbcas12a-d156r entry c…TagsNLSExpressionPlantMutationD156RPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only