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Plasmid#140658PurposeOsTIR1(WT)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(WT) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-FUW-eGFP-RHOA-Q63L
Plasmid#73081PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63LDepositorInsertRHOA-Q63L (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-IDR2
Plasmid#194552PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-CMV-DsRed-IRES-d24EGFP
Plasmid#41944PurposeRetroviral expression of DsRed in mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-eGFP-P62
Plasmid#190929PurposePlasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390DepositorInsertP62 (SQSTM1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (334-449)
Plasmid#87859PurposeFluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationfragment from aa 334 to 449PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP-P2A-mCherry
Plasmid#203868PurposeTet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA
Plasmid#162089PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA
Plasmid#162080PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Otx2-IRES-EGFP
Plasmid#153947PurposepiggyBac transposon vector with CAG promoter expressing Otx2 and EGFPDepositorInsertOtx2 (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Patchless
Plasmid#194553PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-TIGHT-EGFP-donor fw copy
Plasmid#22074DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49235PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-nick sgRNA
Plasmid#214097PurposeLentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3DepositorInsertEGFR L858R nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-nick sgRNA
Plasmid#214098PurposeLentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3DepositorInsertEGFR T790M nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc-EGFP-cre puro
Plasmid#67503PurposeConstitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cellsDepositorInsertluciferase-EGFP-cre
UseCre/Lox, Lentiviral, and Luciferase ; Fluorescent…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-dMQ1-EGFG
Plasmid#89794PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPR and LentiviralTags3*FLAG and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCC; S317A, AGC-->GCCPromoterhUbCAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2
Plasmid#170823PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-EGFP-WPRE
Plasmid#51504PurposeCan be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoterDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135461PurposeMammalian expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-deadBtgh-3NLS
Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionMutationAspartic Acid 242 (in Dennis et al.) changed to A…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
UseTagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…PromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
Plasmid#45929DepositorInsertMCP-2x-yeGFP
UseTags2x-yeGFPExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVQ CMV NanoV1-2a-EGFP ferritin
Plasmid#79649PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS004 - pCAGGS-3XFLAG-CTCF-eGFP
Plasmid#156438PurposeFor transient expression of mouse CTCF tagged with eGFPDepositorInsertmouse CTCF (Ctcf Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mouse PKA-RIalpha-mEGFP
Plasmid#45525DepositorInsertPKA regulatory subunit RI alpha tagged by mEGFP
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mouse PKA-RIIalpha-mEGFP
Plasmid#45527DepositorInsertPKA regulatory subunit RII alpha tagged by mEGFP
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP
Plasmid#194696PurposeHuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_01-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p155 pCMV-EGFP/beta-gal
Plasmid#8387DepositorInsertfloxed EGFP
UseCre/Lox; Beta-galTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-FUW-eGFP-RHOA-T19N
Plasmid#73082PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-T19NDepositorInsertRHOA-T19N (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-eGFP-P2A-dTAG-Ing3
Plasmid#170754PurposeDonor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locusDepositorInserteGFP-P2A-2xHA-dTAG
UseMouse TargetingTagseGFP-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoternoneAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LoxP-mKate-LoxP-EGFP
Plasmid#167940PurposeCMV promoter-LoxP-mKate-LoxP-EGFPDepositorInsertLoxP-mKate-LoxP-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E05: attB2r-meGFP-attB3
Plasmid#162902PurposeGateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p87AAVEGFP hp47phox (S303/304/328D)
Plasmid#112250Purposemutant p47phoxDepositorInsertp47phox (NCF1 Human)
UseAAVTagsEGFPExpressionMutationS303/304/328DPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-EGFP
Plasmid#106964Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only