We narrowed to 25,764 results for: Vit;

1. pcDNA3-hL1

   Plasmid

   #89411

   Purpose

   mammalian expression of hL1

   Depositor

   Fritz Rathjen

   Article

   De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB-Flag-MyoD-E12

   Plasmid

   #25990

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD-E12 Heterodimer Fusion (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yellow Cameleon-Nano140/pcDNA3

   Plasmid

   #51966

   Purpose

   mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano140
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ExRai-AktAR(T/A)

   Plasmid

   #118463

   Purpose

   Negative-control mutant for ExRai-AktAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6T7

   Plasmid

   #71462

   Purpose

   Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

   Depositor

   Willie Mark

   Article

   Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   human U6/phage T7

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-RAB-EKARev(T/A)

   Plasmid

   #118475

   Purpose

   Negative-control mutant for RAB-EKARev biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 GRK2 S670D

   Plasmid

   #45756

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

   Insert

   GRK2 S670D (GRK2 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S670D

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPP5951

   Plasmid

   #128719

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   hopR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK2-miR-34 WT

    Plasmid

    #78258

    Purpose

    psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    fully complimentary miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Circular-ic-CVB3-GFP

    Plasmid

    #226260

    Purpose

    Production of in situ circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6T7G

    Plasmid

    #71463

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    human U6/phage T7

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTXB1-TnY

    Plasmid

    #163975

    Purpose

    IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Peter Smibert

    Article

    Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    TnY
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Circular-ic-EMCV-GFP

    Plasmid

    #226259

    Purpose

    Production of in situ circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge