We narrowed to 13,713 results for: cas9

1. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209781

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   cTNT
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209782

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   sgRNA
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK3258 - human expression plasmid for eSpCas9(1.1)

   Plasmid

   #101176

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K848A/K1003A/R1060A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

   Plasmid

   #91880

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

   Plasmid

   #71213

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
   

   Use

   Tags

   dSpCas9-BFP-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196080

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Blasticidin S deaminase
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

   Plasmid

   #102890

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102892

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

    Plasmid

    #204720

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid

    #109429

    Purpose

    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid

    #121824

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75237

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

    Plasmid

    #188704

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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