We narrowed to 9,921 results for: pET
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Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneTagsTwin StrepExpressionBacterialPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
Tags6X HisExpressionBacterialPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET303-colE1T
Plasmid#155180PurposeExpresses colicin E1 residues 1-190DepositorInsertcolicin E1 residues 1-190
TagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-190PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-colE1TR
Plasmid#155181PurposeExpresses colicin E1 residues 1-364DepositorInsertcolicin E1 residues 1-364
TagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-364PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1deltaART
Plasmid#174804PurposeBacterial expression of an inactive PARP1 mutant lacking the ART subdomainDepositorInsertPARP1deltaART (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 785-1014Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
Tags6XHisExpressionBacterialPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762AAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
Tags6XHis and Tat(48-57)ExpressionBacterialAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-tVYN-Nt∆
Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
ExpressionBacterialPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
TagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
TagsTRX-HisExpressionBacterialPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET11a-dH2A
Plasmid#105500PurposeExpression of Drosophila histone H2ADepositorInsertdH2A
Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2B
Plasmid#105501PurposeExpression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression levelDepositorInsertdH2B
Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ypet-RBD
Plasmid#105291PurposeTo measure Rho activity by binding and FRET analysisDepositorAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET26-CNTnw
Plasmid#100163Purposerecombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target proteinDepositorInsertConcentrative nucleoside transporter
TagsMBP-HisExpressionBacterialPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1CCtoSS
Plasmid#89157PurposeRecombinant expression of PTBP1 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FbGH30
Plasmid#86463PurposeExpression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coliDepositorInsertFbGH30
TagsHis-TagExpressionBacterialPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
Tags6xHis following thrombin cleavage site LVPR|GExpressionBacterialAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only