We narrowed to 162,408 results for: addgene

1. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK 3xFLAG-MTUS1 zeo

   Plasmid

   #192323

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-MTUS1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   MTUS1 (MTUS1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dopey2_pCSdest

   Plasmid

   #53850

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   Dopey2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. LoriT

   Plasmid

   #163691

   Purpose

   Modified L4440 containing now CamR and oriT for conjugation

   Depositor

   Baris Tursun

   Article

   Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

   Insert

   oriT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGEN-SBP-DICER1

   Plasmid

   #50558

   Purpose

   purification of Dicer1 from mammalian cells

   Depositor

   Yukihide Tomari

   Article

   Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

   Insert

   Dicer1 (Dicer1 Mouse)
   

   Use

   Tags

   SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBabe-puro-miR-10b sponge

   Plasmid

   #25043

   Depositor

   Bob Weinberg

   Article

   Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

   Insert

   miR-10b sponge
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pLentiCRISPRv1.mir451_KO

   Plasmid

   #164689

   Purpose

   For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   miR-451 CRISPR KO gRNA (MIR451A )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2385

    Plasmid

    #36794

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Right (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pIS1 Cyclin D2 long UTR

    Plasmid

    #21645

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long UTR (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.pCry1(408).DIO.Luciferase

    Plasmid

    #192623

    Purpose

    Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

    Depositor

    Michael Hastings

    Article

    Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

    Insert

    luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG260

    Plasmid

    #33210

    Depositor

    David Bartel

    Article

    Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

    Insert

    SYNM (SYNM Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early enhancer/promoter

    Available since

    Jan. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. CMVTCRa_candidate5

    Plasmid

    #164999

    Purpose

    Expression of candidate TCR #5 TCRalpha cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRAV (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMVTCRb_candidate5

    Plasmid

    #165000

    Purpose

    Expression of candidate TCR #5 TCRbeta cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRB (TRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_TMv2

    Plasmid

    #131761

    Purpose

    frameshift reporter for barcode retrieval

    Depositor

    Paul Blainey

    Article

    Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX304 LacZ-V5 blast

    Plasmid

    #83099

    Purpose

    Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2x V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pCRUSH

    Plasmid

    #54480

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pOZ-Pax3-FH-C-puro

    Plasmid

    #32517

    Depositor

    Andrew Lassar

    Article

    Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

    Insert

    Pax3 (Pax3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. CRISPR_TRBC

    Plasmid

    #164994

    Purpose

    Expression of gRNA targeting TCRbeta constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRBC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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