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pRS415-TEF1pr-SEC63-FAPoptim

Plasmid

#221116

Purpose

FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim-SEC63

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_174_neoR

Plasmid

#228935

Purpose

Lentiviral expression of EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_052_HygroR

Plasmid

#228937

Purpose

Plasmid for cloning of custom gRNA using EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC61-FAPoptim

Plasmid

#221115

Purpose

FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-MFA-NFAPoptim

Plasmid

#221095

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-MFA-NFAPoptim

Plasmid

#221090

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SNAP-CD86tm-eGFP-FKBP(f36v)

Plasmid

#200280

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAP-tag-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)

Plasmid

#200281

Purpose

Membrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtag

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

eGFP-CD86tm-HaloTag-FKBP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-10His-FKBP(f36v)-SNAP

Plasmid

#200282

Purpose

For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

FKBP-SNAPtag

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Use

Tags

Expression

Mutation

Promoter

smCBA

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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2BT-His-TEV-cs-LC3b

Plasmid

#197460

Purpose

Bacterial Expression of human LC3b

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

LC3b (MAP1LC3B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2371

Plasmid

#225637

Purpose

High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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px552-sg-gria1-HT

Plasmid

#187652

Purpose

Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al eLife. 2024 Aug 15;13:e80622. doi: 10.75

Inserts

HaloTag donor sequence
miRFP670

Use

AAV and CRISPR

Tags

N/A

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM1

Plasmid

#222924

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

PRDM1 (PRDM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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