We narrowed to 7,333 results for: ef1a;

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pLV-TET1-dCas9

Plasmid

#235599

Purpose

Expresses Tet1CD-dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xNLS and TET1 CD

Expression

Mammalian

Promoter

EF1a

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN11

Plasmid

#215523

Purpose

Expression vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11-Clover

Plasmid

#215525

Purpose

Expression vector with clover-tagged Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

Plasmid

#223146

Purpose

Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

Plasmid

#223156

Purpose

Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

Plasmid

#204622

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

mRFP1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_HA-C7orf26v2

Plasmid

#178536

Purpose

Subcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v2 (INTS15 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_HA-C7orf26v1

Plasmid

#178535

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A

Plasmid

#175140

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C211A

Plasmid

#175139

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C211A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Hsd11b1-V5

Plasmid

#175147

Purpose

Lentiviral expression of mouse Hsd11b1-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Hsd11b1 (Hsd11b1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMCh-633

Plasmid

#92253

Purpose

Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-Y89XF

Plasmid

#84559

Purpose

mammalian expression of TSAd-Y89XF

Depositor

Anne Spurkland

Article

Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

Y279F, Y290F, Y305F

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
L40C-CRISPR.EFS.mNeon

Plasmid

#69146

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.tRFP

Plasmid

#57819

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ENL)

Plasmid

#69212

Purpose

Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-GAPGlySer-PDGFR

Plasmid

#240247

Purpose

Lentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TM

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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