We narrowed to 4,374 results for: NAP

1. RhoC gRNA #1

   Plasmid

   #198724

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RhoC gRNA #2

   Plasmid

   #198725

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RhoC gRNA #3

   Plasmid

   #198726

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30-DARPin-E11

   Plasmid

   #226784

   Purpose

   Expression of His-tagged DARPin E11 in E. coli

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   DARPin E11
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px552-sg-gria1-HT

   Plasmid

   #187652

   Purpose

   Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Inserts

   HaloTag donor sequence
   miRFP670
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. opt.a_pSTC0-zeo

   Plasmid

   #202347

   Purpose

   Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   opt.a
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. opt.b_pSTC0-zeo

   Plasmid

   #202348

   Purpose

   Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   opt.b
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. neg.ctrl_pSTC0-zeo

   Plasmid

   #202350

   Purpose

   Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   neg. ctrl
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-DIO-GCaMP6f-WPRE

   Plasmid

   #216275

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human Synapsin 1 (hSyn)

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-syn-F14F15S-jGCaMP7s

    Plasmid

    #178514

    Purpose

    AAV vector for Flpo recombinase dependent jGCaMP7s

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    jGCaMP7s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p202-Syt1-C2A-wt

    Plasmid

    #201553

    Purpose

    Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Synaptotagmin-1 C2A domain (partial)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli

    Promoter

    RBS

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX SYT9 C2B D330, 332N

    Plasmid

    #195705

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 253-344 of mouse SYT9 with D3…

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX SYT9 C2AB D197,199,330,332N

    Plasmid

    #195706

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9 with D1…

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-TRE-IEo

    Plasmid

    #201992

    Purpose

    AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Tight TRE

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEntry_HCoV-229E-nsp4

    Plasmid

    #168953

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp4 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEntry_HCoV-229E-nsp6

    Plasmid

    #168955

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp6 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEntry_HCoV-229E-nsp9

    Plasmid

    #168958

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp9 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEntry_HCoV-229E-nsp10

    Plasmid

    #168959

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp10 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEntry_HCoV-229E-nsp14

    Plasmid

    #168960

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp14 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEntry_HCoV-229E-nsp15

    Plasmid

    #168961

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp15 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge