We narrowed to 8,294 results for: construct

1. PlayBack-U6-Not1

   Plasmid

   #203301

   Purpose

   For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PlayBack-CMV-Bamh1

   Plasmid

   #203303

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PlayBack-CMV-Nhe1

   Plasmid

   #203304

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PlayBack-CMV-EcoR1

   Plasmid

   #203305

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PlayBack-CMV-Bgl11

   Plasmid

   #203306

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PlayBack-Ef1a-Nde1

   Plasmid

   #203309

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PlayBack-Ef1a-Nhe1

   Plasmid

   #203310

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PlayBack-U6-Kpn1

   Plasmid

   #203297

   Purpose

   For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAN1 gRNA HIS

   Plasmid

   #182521

   Purpose

   Expression vector for gRNA directed against CAN1 ORF.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   CAN1 gRNA (CAN1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C-SWAT SMASh

    Plasmid

    #182523

    Purpose

    C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    SMASh Tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-SWAT 3MYC SMASh

    Plasmid

    #182524

    Purpose

    C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    SMASh Tag
    

    Use

    Tags

    3MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C-SWAT 3MYC SMASh

    Plasmid

    #182525

    Purpose

    C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    SMASh Tag
    

    Use

    Tags

    3MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C-SWAT GFP DAmP

    Plasmid

    #182511

    Purpose

    C-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    GFP
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYCTK010

    Plasmid

    #176714

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ka
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYCTK006

    Plasmid

    #176710

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    P-SST2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p{Mhc-F4-678-EGFP::GW,attB}

    Plasmid

    #182170

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p{Mhc-F4-678-DsRed::FRT-RC,attB}

    Plasmid

    #182187

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p{Mhc-F4-678-GFP::KD-RC,attB}

    Plasmid

    #182184

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p{Mhc-F4-678-DsRed::FRT,attB}

    Plasmid

    #182186

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p{Mhc-F4-678-DsRed::KD-RC,attB}

    Plasmid

    #182189

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge