We narrowed to 3,422 results for: mpo

1. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

   Plasmid

   #159696

   Purpose

   E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
   

   Use

   Tags

   His6-Halotag-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

   Plasmid

   #159698

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-EF-FH-TAZ S89A-ires-blast

   Plasmid

   #52084

   Purpose

   Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ S89A (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   S89A

   Promoter

   EF1a

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

   Plasmid

   #216480

   Purpose

   lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   TAZ4SA (WWTR1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   S66A, S89A, S117A, S311A

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV TRE3G Neo GFP-Lamin A wildtype

   Plasmid

   #118709

   Purpose

   Lentiviral TET-ON inducible GFP-Lamin A wildtype

   Depositor

   Tom Misteli

   Article

   Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

   Insert

   GFP-Lamin A human wildtype (LMNA Human)
   

   Use

   Lentiviral; Tet-on inducible

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G (TET-ON)

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-EF-FH-TAZ-ires-blast

   Plasmid

   #52083

   Purpose

   Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-N-Flag-NLRP3 711-1034

   Plasmid

   #75141

   Purpose

   Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 711-1034

   Promoter

   CMV

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid

    #169161

    Purpose

    To express nsp10-nsp14 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-NanoLuc-BRAF-CAT

    Plasmid

    #194772

    Purpose

    Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
    

    Use

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-BRAF-REG-Halo

    Plasmid

    #194771

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R725-X11-566: His6-MBP-tev-Hs.LGALS3

    Plasmid

    #159699

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.LGALS3

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti CMV TRE3G Neo GFP-Progerin C661S

    Plasmid

    #118711

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin C661S (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C661S farnesylation mutant

    Promoter

    CMV TRE3G (TET-ON)

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p-Tet-O-RUNX1T1-Puro

    Plasmid

    #221666

    Purpose

    Expresses RUNX1T1 in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    RUNX1T1 (RUNX1T1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

    Plasmid

    #159700

    Purpose

    E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge